papain extraction industry

papain extraction industry
Author: EIRI Project Consultants
ISBN: project report
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                                                                  PAPAIN EXTRACTION INDUSTRY

The papaya is the fruit of the papaya tree (Carica papaya) native of Central America.The fruit ripens from 4 to 6 months depending on the climate where it is grown (Salunkhe and Kadam, 1995). The cultivation of this fruit has two main purposes: the sale of the fruit for human consumption and the extraction of enzymes that constitute 40% of the latex in 1 mM concentrations. The papain is a natural proteolytic enzyme that is extracted from the latex in the leaf, the stem and the papaya's unripe fruits. Papain is used in a many industrial fields (like pharmaceutical, brewery, meat, dairy, textile, photographic, optical, tanning, cosmetic, detergents, food and leather industry), because a synthetic enzyme is not capable of simulating the properties of the natural enzyme, which increased its demand. The process to obtain raw papain consists of two main stages: latex extraction and drying. A third stage, purification, may be used if a purified papain is wanted. This work compares the crude enzymatic activity obtained from locally Carica papaya using the unripe fruit and the skin juice under different drying processes and evaluates the enzyme activity for the proposed purification procedure.

     A common enzyme known as papain is obtained from the green papaya (pawpaw) fruit. Enzymes are proteins that can increase the rate of biological changes such as the ripening of fruit. At  the end  of  an  enzyme  catalysed  reaction  the  enzyme  itself  is unchanged and is able to react again.


     Enzymes can be generally recognized by the ending -ase. This either indicates  the nature of the substance  affected  by  the enzyme  (e.g.  carbohydrase  acts on  carbohydrate  material  and proteases  acts  on proteins) or to indicate the  nature  of  the reaction  e.g.  transferases catalyse the transfer  of  atoms  or groups of atoms within a substance.

                                                                  Cost Estimation              

Plant Capacity                                                                        : 100.Kgs/Day
Land and Building Required ( 3 Acrer )                              : Rs.    90.00 Lacs
Plant & Machinery                                                                   : Rs. 82.00 Lacs
Working Capital for  3 Months                                                : Rs. 61.00 Lacs
Total Capital Investment                                                         : Rs. 2.44 Cr
Rate of Return                                                                          : 46%
Break Even Point                                                                     : 48%

MARKET SURVEY CUM DETAILED TECHNO
ECONOMIC FEASIBILITY REPORT covers

 
•            Introduction
•            Properties
•            BIS (Bureau of Indian Standard) Specifications & Requirements
•            Uses & Applications
•            Present Indian Market Position
•            Expected Future Demand
•            Export & Import Statistics Data
•            Names and Addresses of Existing Units (Present Manufactures)
•            List of Plant & Machineries
•            Miscellaneous Items and Accessories
•            Instruments, Laboratory Equipments and Accessories
•            Electrification, Electric Load and Water
•            Maintenance, Suppliers/Manufacturers of Plant and Machineries
•            Process of Manufacture with formulae if applicable
•            Flow Sheet Diagram
•            List of Raw Materials
•            Availability of Raw Materials
•            Requirement of Staff & Labour
•            Personnel Management
•            Skilled & Unskilled Labour
•            Requirement of Land Area
•            Built up Area
•            Plant Layout.
 
along with financial details as under:
 
Summary of Capital Cost of Project
Land & Side Development Exp.
Buildings
Plant & Machineries
Misc. Fixed Assets
Technical Know how Fees & Exp.
Preliminary Expenses
Pre-operative Expenses
Provision for Contingencies
below mentioned financial statements (Annexure) will be  for 5 to 10 Years
  Annexure  ::         Cost of Project and Means of Finance
  Annexure  ::         Output, Profitability and Cash Flow Chart
  Annexure  ::         Assessment of Working Capital requirements
  Annexure  ::         Sources of Finance
  Annexure  ::         Balance Sheets
  Annexure  ::         Break-Even Analysis and profitability analysis.
  Annexure  ::         Quantitative Details-Output/Sales/Stocks
  Annexure  ::         Sales Realisation        
  Annexure  ::         Raw Material Cost       
  Annexure  ::         Other Raw Material Cost             
  Annexure  ::         Packing Material Cost         
  Annexure  ::         Consumables, Store etc.,                    
  Annexure  ::         Employees Expenses            
  Annexure  ::         Fuel Expenses                 
  Annexure  ::         Power/Electricity Expenses    
  Annexure  ::         Repairs & Maintenance Exp.    
  Annexure  ::         Other Mfg. Expenses           
  Annexure  ::         Administration Expenses       
  Annexure  ::         Selling Expenses              
  Annexure  ::         Depreciation Charges - Profitability
  Annexure  ::         Depreciation Charges
  Annexure  ::         Interest and Repayment - Term Loans
  Annexure  ::         Tax on Profit
  Annexure  ::         Assumptions for Profitability workings
  Annexure  ::         Assessment of Working Capital

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