Manufacturing of Tea, Tea Processing, Blending, Chemical Composition, Nutritional Value, Medicinal Uses with Packaging Technology

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Manufacturing of Tea, Tea Processing, Blending, Chemical Composition, Nutritional Value, Medicinal Uses With Packaging Technology

The Book contains the chapters: Antioxidant Compounds of Tea: Effect of Processing and Storage, The Cytogenetic Effects of Black Tea and Green Tea, Green Tea Polyphenols Block the Anticancer Effects of Bortezomib and other Boronic Acid, HPLC Analysis of Commercial Teas and Green Tea, Green Tea As A Phyto-Therapeutic Agent In Medicine And Dentistry, Chemical Composition of Tea, Green Tea (Camellia sinensis L.) leaves, Medicinal and therapeutic potentialities of tea (Camellia sinensis L.), Green Tea Polyphenols, Nutritional Value Of Dried Tea Leaves and Infusions of Black, Green and White Teas, Medicinal Uses for Herbal Teas, Tea Manufacture and Quality Improvement via Tea Machinery Management, Tea blending unit, Processing and Marketing of Tea, Packaging of Tea, Tea Processing Industry.

Preface

Tea is one of the most popular beverages that are being consumed all over the world. Tea is known as a soothing drink and a way of life. Owing to its increasing demand, tea is considered to be one of the major components of world beverage market. Tea is very beneficial for health and is also known as anticarcinogenic properties. Green tea acts as an antiviral agent. Growing tea requires sufficient amount of work and there is additional level of work that must be incorporated to harvest it. Tea is cultivated in tropical and sub tropical regions. There are various kinds of tea such as black tea, green, white tea that can be obtained from real tea plant, Camellia sinensis. The making of different varieties of tea mainly depends upon plucking and rolling, spreading, storing process.

Black tea occupies the majority part of world tea production. The most important features of black tea manufacture involve different operations like - plucking and handling of green leaves, withering, processing (CTC), Fermentation, drying, sorting and packing. Each stage involves characteristic changes in the physical and biochemical composition of the leaves. Quality of made tea depends on leaf standard, making of high quality tea depends very largely on the fineness of the leaf. Any physical damage to plucked leaves, i.e. distortion or overheating will instantly initiate chemical reactions which may impair the quality of the made tea. Therefore, careful handling of green leaves from field to factory is pre-requisite to obtain good tea. There is a statement "Good teas are made in the withering house" Withering plays a major role particularly in CTC manufacturing contributing significantly to quality. So our utmost effort must be there to achieve correct degree of weather. Processing is the step of manufacturing where distortion of withered leaves is done. The aim is to increase permeability of leaves cell wall and to mix up different compound in the leaves so that oxygen can get through cell wall and help in oxidation. Fermentation of tea leaf is the process of oxidation of various chemical constituents of tea leaf through a series of chemical reactions. Colour, flavour, strength and briskness are developed in this stage.

The book describes aspects of tea cultivation, ranging from the history of old crop, machinery & equipment for various Tea, biological control, organic tea- and many more. This is a sincere attempt to open up the world of this wonderful beverage, its cultivation methods, types of tea available worldwide, manufacturing process, to the common man.

Some of the fundamentals of the book are growth of tea in other countries, tea in Indian economy, biochemical constituents, pharmacological properties, selection, pollination and propagation, nutritional requirements, growth, photosynthesis and respiration, nursery management, water theory, oxidative degradation of protein, biological effect of polyphenols, analysis of tea, tea processing, green tea processing, tea bag production etc.

This book will be a mile stone for its readers who are new to this sector, will also find useful for entrepreneurs, tea scientists and tea research establishments.

Contents

Antioxidant COMPOUNDS of Tea: Effect of Processing and Storage

Introduction
Antioxidant Compounds of Tea
Flavan--ols
Phenolic Acids
Flavonols and Flavones
Theaflavins and Thearubigins
Theasinensins
Theabrownins
Effect of Production and Processing
Phenolic Compounds in Fresh Tea Leaves
Manufacturing Process
Withering
Steaming/Pan-Firing
Effects of Production and Processing
Rolling
Oxidation/Fermentation
Drying
White Tea
Yellow Tea
Green Tea
Oolong Tea
Black Tea
Pu-erh Tea
Home Preparation of Tea Infusions
Water Quality
Temperature and Time
Tea Leaves/Tea Bags/Tea Powder
Serving Additives
Production of Ready-to-Drink Tea Beverages
Tea Leaves/Tea Bags/Tea Powder
Serving Additives
Production of Ready-to-Drink Tea Beverages

The Cytogenetic Effects of Black Tea and Green Tea

Introduction
Material and Methods
Preparation of aqueous extracts
Chemicals
In vitro mitotic index assay
In vitro micronucleus assay
Statistical analysis
Results
Micronucleus
Mitotic index
Replication index
Discussion

Green Tea Polyphenols Block the Anticancer

Effects of Bortezomib and other Boronic Acid

Introduction
Methods
Materials
Cell lines and culturing
MTT assay
Colony-formation assay
Apoptosis measurements
Immunoblots and antibodies
Proteasome activity assay
Alizarin red S assay
Drug treatment of nude mice
Hematoxylin and eosin staining
Results
EGCG blocks the cytotoxic effects of BZM in glioblastoma cell lines
EGCG differentially affects cytotoxicity of
various proteasome inhibitors
EGCG prevents proteasome inhibition and ER
stress induction when combined with boronic acid-based compounds
EGCG neutralizes the antitumoral effects of BZM in vivo
EGCG inactivates BZM via a direct reaction with its boronic acid group

HPLC Analysis of Commercial Teas and Green Tea

Introduction
Materials and Methods
Materials
Extraction and analysis of commercial tea leaves and
green tea dietary supplements
Statistical analysis
Results and Discussion
Analytical aspects
Composition of tea leaves
Composition of dietary green tea supplements

Green Tea As A Phyto-Therapeutic Agent In Medicine And Dentistry

Introduction
Pharmacological Uses of Green Tea in the
Field of Medicine
Role of Green Tea in the Field of Dentistry
Green Tea: Not Everything is Positive

Chemical Composition of Tea

Introduction
Primary Compounds Present in Tea and Their
Structural Formulas
Chromatographic Methods Used to Determine
the Chemical Composition of Tea
Determination of Volatile Impurities in Tea by
Gas Chromatography
Determination of Amino Acids and Sugars in Teas
by Anion Exchange Chromatography
Application of High Performance Liquid
Chromatography (HPLC) for the Determination
of non-Volatile Compounds in Tea
Analytical Methods
Analyte Extraction
Determination of Non-Volatile Compounds

Green Tea (Camellia sinensis L.) leaves

Introduction
Materials and Methods
Result and Discussion

Medicinal and therapeutic potentialities of tea

Camellia sinensis L.
Introduction
Tea, the chemical constituents and
activities of polyphenols
Tea, various types with varying properties
Health benefits of tea consumption on human
beings
Cognitive functions with tea consumption

Green Tea Polyphenols

Introduction
Setbacks related to the use of green tea polyphenols
Nano-approaches for improved oral bioavailability
and efficacy of green tea polyphenols

Nutritional Value Of Dried Tea Leaves and

Infusions of Black, Green and White Teas

Introduction
Materials And Methods
Result And Discussion

Medicinal Uses for Herbal Teas

Medicinal uses for common herbs
How do I know if tea is right for my patient?
Tea Preparation
Where do my patients get teas or herbs?
How long will tea last?

Tea Manufacture and Quality Improvement

Vis-à-vis Tea Machinery Management

Introduction
Specification for Black Tea- ISO Standard
Manufacturing of black tea
Plucking leaf standard
Effect of coarse plucking
Leaf Handling
Analysis of green leaf
Withering
Benefit of withering
CTC
Sharpening of CTC rollers
Fermentation
Drying
Sorting
Conclusion

Tea blending unit

Introduction
Product & Its Application
Desired Qualifications For Promoter
Raw Material Requirements
Manufacturing Process

Processing and Marketing of Tea

Processing by small farmers
Processing for green tea
Storage between plucking and processing
Fermentation stopping
Rolling (sometimes called "squeezing")
High temperature drying
Low temperature drying (and scenting if desired)
Classification
Final roasting
Packaging and labeling
Customers within Viet Nam
Customers in other countries

Packaging of Tea

Modular System For Maxim
Compacta Tag (With Hand Removal System)
Compacta Tag (With Automatic Box Fi Lling System)
Compacta Hs (With Hand Removal System)
Compacta Hs (With Automatic Box Fi Lling System)

Tea Processing Industry

Soil
Rainfall
Humidity
Air Temperature
Land Preparation
Erosion Control And Drains
Water Requirement
Soil Chemistry
Fertilizer
Primary nutrients
Secondary nutrients
Micronutrients
Phosphorus (P)
Potassium (K)
Secondary nutrients : Calcium (Ca)
Magnesium (Mg)
Sulphur (S)
Phosphorus (P)
Potassium (K)
Secondary nutrients : Magnesium
Sulphur
Micronutrients : Boron
Organic Fertilizer
Green Manure
Manure from plants in the field
Electrical Power Source

References

List of Tables

Table An Overview of the Studies Carried Out on the Phenolic Compounds Composition of Different Types of Tea

Table - Mitotic index (%) (mean ± SDs) in human lymphocyte cultures exposed to extracts of black tea and green tea.

Contents

Table . --- Content of catechins [(–)-epigallocatechin (EGC), (–)-catechin (C), (+)-epicatechin (EC), (–)-epigallocatechin--gallate (EGCG), (?)-gallocatechin--gallate (GCG), (?)-epicatechin--gallate (ECG), and (–)-catechin--gallate (CG)], their sum (total CATS); theaflavins [theaflavin (TF), theaflavin--gallate (TFG),theaflavin-_-gallate (TF_G), and theaflavin-,_-digallate (TF_G)], their sum (total TFS); sum of catechins and theaflavins (total flavonoids, FLAV); caffeine (CAF), theobromine (TB), and theophylline (TP), their sum (total alkaloids) in black, green, specialty, and herbal teas extracted with % ethanol/water (E) or water (W)

Table . Commercial green tea extracts sold as capsules, gums, and powders

Table . Primary chemical compounds present in tea (analyzed by Chromatographic Methods)

Table . Chromatographic methods used for the chemical analysis of tea

Table .. Classes of compounds contained in aroma of black tea

Table .. Main volatile components determined in tea samples

Table .. Content of Sugars in Green Tea Determined by HPLC with Amperometric Detector

Table .. Content of amino acids in green tea determined by HPLC with pulsed amperometric detector

. Application of High Performance Liquid Chromatography (HPLC) for the Determination of non-Volatile Compounds in Tea

Table .. Extraction of polyphenols from tea using different solvents (mg/g)

Table .. Content of flavonoids (% dry weight) in the leaves of green tea, oolong tea, and black tea

Table .. Content of catechins, gallic acid, caffeine, theophylline, and theobromine in green tea, oolong tea, black tea, and pu-erh tea, in mg/g

Table .. Content of catechins, gallic acid, and caffeine in various tea grades

Table . Content of catechins and caffeine in various teas determined by HPLC-MS

Table . Content of polyphenols in green teas from Japan, oolong tea from China, and black tea from Kenya, in mg/g

Table .. Content of epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate (ECG), catechin (C), epicatechin (EC), and total amount of all catechins in various grades of tea from China, Taiwan, and Japan, in mg/g of dry tea (W - hot water extracts, A - alcoholic extract, %)

Table .. Total polyphenols, total catechins and caffeine content in tea

Table .: Differences among green and black teas

Table .a: Effect of consumption of tea on combating different types of cancer

Table .b: Effect of consumption of tea on combating different types of cancer.

Table .: Content of caffeine (mg) in a seven cup in regular soft drinks.

Table .: Polyphenolic components in green and black tea (% w/w of extract solids).

Table . Mean values and standard deviations in the analysis of chemical composition in Chinese black teas.

Table . Mean values and standard deviations in the analysis of chemical composition in Chinese green teas

Table .. Mean values and standard deviations in the analysis of chemical composition in Chinese white teas

Table .. Mean values and standard deviations in the analysis of selected mineral elements and caffeine contained in infusions of Chinese black teas

Table .. Mean values and standard deviations in the analysis of selected mineral elements and caffeine contained in infusions of Chinese green teas

Table .. Mean values and standard deviations in the analysis of selected mineral elements and caffeine contained in infusions of Chinese white teas

Table .. The content of nutrition components, minerals and caffeine in different types of Chinese tea

Table .. Coefficients of correlation between the contents of protein, caffeine and mineral elements in infusions of black,green and white teas

Table Chemical requirements for black tea

Table Analysis of green leaf by counting

Table . Physical wither of leaf needed with different fresh leaf moisture content

Table . Parameter of chemical withering

Table . Requirement of milling and chasing groove depth for standard chasing

Table . Conditions for different type of fermentation

Table . Drying condition of different dryer

Table . Different sorting machine

Table . Some of the minimum physical and chemical standards for green tea, as established by Vietnamese Standards Bureau.

Table . Indicators for classifying green tea into quality grades, as established by Vietnamese Standards Bureau. The weights given to the various indicators: appearance %, colour of liquor %, smell %, taste %.

Table .: General Chemical Properties of some Tea Soils of the World

Table .: Physical Properties of some Tea Soils of the World (- cm depth on average)

Table - . : Nutrient Content in Commercial Fertiliser used in Tea

Table - . : Relative distribution of chemical compounds in the tea bush

Photograph - . : Picture shows diesel fired burner attached with the drier. Fermented tea is dried at °C - °C.

Table-.: Description & Characteristics of CTC Tea

Photograph - . : Grading of Orthodox Tea (Makai Bari).

Table- .: Thermal Energy Consumption of Different Energy Sources

Table- . : Section-wise energy Consumption in Typical CTC Factory

Table-.: Pests Of Tea

Table-.: Sequence in the Appearance of Different Kinds of Arthropod Pests on Tea

Table - .: Classification of Pesticides based on

Target Pests

Table-.: Some details of Available Organic and Inorganic Pesticides

Table - .: Pesticides and their Recommended Dosage

Table-.: Steps for Effective Control of Pests

Table - .: Safety Precautions Needed for Application of Pesticides

List of figures

Figure Chemical structure of theogallin. Theogallin and gallic acid are the most common simple phenolics in tea.

Figure Mechanism of theaflavin formation. Theaflavins are formed as a result of co-oxidation of selected pairs of catechins catalyzed by polyphenol oxidase (PPO). R = H - EGC, R = galloyl group - EGCG, R = R = H - theaflavin; R = galloyl group, R = H and R = H, R = galloyl group - theaflavin gallate; R = R = galloyl group - theaflavin digallate.

Figure Chemical structure of theanisenisins: theanisenisin A: R = R = galloyl group; theanisenisin B: R = galloyl group, R = H and R = H, R = galloyl group; theanisenisin C: R = R = H.

Figure Flow chart of processing of different types of tea: white, yellow, green, oolong, black CTC, crush-tear-curl.

Figure Flow chart of processing of different types of pu-erh tea.

Figure - Percentage change in lymphocyte micronuclei, according to the control and treatment with different concentrations of green tea and black tea. MN rates were decreased by the extracts of both the black tea and green tea. While these decreases were dose-dependent and statistically significant for the black tea, they did not significant for green tea. The MN rates of the control and the black tea extracts (., , , and mg/mL) were , , ,, and %, respectively. The MN rates of the control and green tea extracts (., , ,and mg/mL) were , , , , and %, respectively. The MN rates ( and %) of the and mg/mL extracts of the black tea were quite lower than the control (%).

Figure - Replication index changes in the lymphocyte, according to the control and treatment with different concentrations of the green tea and black tea. The RI rates were increased by the extracts of both the black tea and green tea. But these increases were not statistically significant. The RI rates of control and black tea extracts (., , , and mg/mL) were , , , , and , respectively. The RI rates of the control and green tea extracts (., , , and mg/mL) were , , , , and , respectively. The most RI values were mg/mL concentration for the black tea and mg/mL concentration for the green tea.

Figure . EGCG neutralizes the cytotoxic effects of BZM in multiple myeloma cells. (A) Chemical structures of Bortezomib

Figure . EGCG neutralizes the cytotoxic effects of BZM in glioblastoma. (A) Percentage cell viability of LN cells treated for hours with increasing concentrations of BZM in the presence of increasing concentrations of EGCG was determined via MTT assay. (B) U and LN were treated with nM BZM in the presence or absence of or _M EGCG for hours and the number of long-term surviving cells ( weeks) was determined by colony-formation assays. Images were taken of representative -well plates. (C) The average number of colonies of U and LN cells treated with BZM in the presence or absence of EGCG is presented as the percentage of colonies from untreated cells, which was set at % (mean _ SE; n _ ).

Figure .. Complete green tea extract and its polyphenols EGC, ECG, and EC neutralize the cytotoxic effects of BZM.

Figure .. EGCG neutralizes the cytotoxic effect of MG- and proteasome inhibitor IX but not NFV,MG-, and proteasome inhibitor I. (A) Chemical structures of the proteasome inhibitors NFV, proteasome inhibitor I (PS-I), MG-, MG-, and proteasome inhibitor IX (PS-IX), and their grouping according to the presence of a boronic acid. (B-F) LN cells were treated with increasing concentrations of the individual proteasome inhibitors in the presence or absence of _M EGCG for hours; thereafter, the number of long-term surviving cells that were able to spawn colonies during the following weeks (in the absence of drug treatment) was determined by colony-formation assays.Shown is the mean plus or minus SE (n _ ). (G) Typical result of MTT survival assay of LN cells treated for hours with increasing concentrations of BZM in the presence of increasing concentrations of either MG- or MG- Shown are wells from a -well plate. Deep purple colour represents fully viable cells; pink(ish) colour indicates reduced viability; yellow indicates absence of viable cells.

Figure .. EGCG prevents proteasome inhibition and ER stress induction when combined with BZM and MG-, but not MG- (A) LN cells were cultured for hours with nM BZM, _M MG-, or nM MG- in the presence or absence of _M EGCG. (Top) Percentage proteasome activity in total cell lysates was determined via S proteasome activity assay. (Bottom) The relative amount of polyubiquitinated proteins and the ER stress marker GRP was determined via Western blot analysis with specific antibodies against ubiquitin and GRP, respectively.Western blot to actin was used as a loading control. (B) RPMI/ cells were cultured for hours with nM BZM in the presence or absence of _M EGCG. (Top) Percentage proteasome activity from total cell lysates was determined via S proteasome activity assay.(Bottom) The relative amount of polyubiquitinated proteins, activated caspase- (cleaved casp-), and cleavage of poly (ADP ribose) polymerase (PARP, which indicates ongoing apoptosis) in RPMI/ cells was determined via Western blot analysis with specific antibodies against ubiquitin, caspase-, and PARP, respectively. (C) Highly purified proteasomes were treated with increasing concentrations of BZM in the presence or absence of _M EGCG. Percentage activity was determined via S proteasome activity assay. Shown is percentage proteasome activity (mean _ SE; n _ ), where activity in the absence of drug was set to %.

Figure .. EGCG neutralizes the antitumoral effects of BZM in multiple myeloma in vivo. Tumor-bearing mice

Figure . Eight major catechins found in tea

Figure . Structure formula of tea theoflavins

Figure .. Structure of oligomeric catechins

Figure .. Structure formulas of tea Theanine and ?-Aminobutyric acid (GABA)

Figure .. Chromatogram of green tea (brewing time min) on a x.mm column with C Luna (-) eluted with acetonitrile and water; - (-)- gallocatechin (GC), - (-)- epigallocatechin (EGC), - (-)- epicatechin (EC), - (-)- epigallocatechin gallate (EGCG), - (-)- epicatechin gallate (ECG)

Fig. . Chemical structure of major catechins in green tea. EC, (-)-epicatechin; ECG, (-)-epicatechin--gallate; EGC, (-)-epigallocatechin; EGCG, (-)-epigallocatechin--gallate.

Fig. (a): moisture percentage of green leaf, withered leaf and made tea during tea processing

Fig. (b): moisture percentage of green leaf, withered leaf and made tea during tea processing

Fig. : Energy saving during withering and drying

Typical tea drying drum. Problems include that drum is made of low-quality steel, and fire is only partly enclosed so heat is not evenly distributed.

Fig: .

Photograph - . : Basket carrying green tea leaves for further processing to black tea. System of transportation observed with small gardens. Eco-friendly transport system.

Photograph-. : View of plantation in Darjeeling. Portion has already been plucked.

Photograph - . : Solid waste from tea garden after deep pruning. This solid waste acts as a fuel in rural area.

Photograph - . : Makai Bari Tea Gardens. Produces % bio-dynamic tea. No chemical/pesticides used here.

% EUU.

Photograph . Rotorvane machine. Parnal cutting, turning and curling operation take place in this machine (CTC)

Photograph . CTC rollor complete cutting, turning and curling operation takeplace in CTC roller after totorvance operation.

Photograph . Gugi machine make CTC tea of current formula

Photograph . Orthodox roller rolls the weathered leaf conntinuously in the

Figure-.: Ctc Tea Manufacturing - Process Flow

Diagram

Figure-.: Orthodox Tea Manufacturing - Process Flow Diagram

Figure-.: Green Tea Tea Manufacturing - Process Flow Diagram

Fig .. :Flow Diagram of the Mass Balance

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